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by two studies, both conducted by the US Centers for Disease Control and Prevention (CDC). The annual Behavioral Risk Factor Surveillance System (BRFSS), which uses self-reported data from telephone interviews, estimated the prevalence of obesity among adult Americans in 2006 to be 25% (Yun et al., 2006). By contrast, the more rigorous National Health and Nutrition Examination Survey (NHANES) reported that 33% of American men and 35% of women were obese in 2003 2004 (National Health and Nutrition Examination Survey, 2003). (NHANES examines a strati ed probability sample of the population, who are interviewed and examined in a standardized fashion by trained staff.) It is clearly important for decision-makers to use accurate data when setting priorities and allocating resources in healthcare (Connor Gorber et al., 2007). Another problem is the time taken to collect and process data. Some reports are based on information that may be years out of date a potentially important consideration when the incidence of obesity is rising so rapidly. Across European countries, surveys of adult overweight and obesity are inconsistent in their timing, sample size and methodology, with several countries relying heavily on self-reported data (see Table 2.2). The International Obesity Task Force (IOTF) has highlighted the need for rigorous and systematic surveillance of obesity throughout Europe, including robust measurements of height, weight and waist circumference (International Obesity Task Force, 2005). As a result, the European Commission has established the European Health Interview Survey (EHIS) to standardize the collection of self-reported data on height, weight, physical activity and consumption of fruit and vegetables. This will be followed in 2010 by the European Health Examination Survey (EHES), introducing objective measures of height, weight, blood pressure and cholesterol in a randomly-sampled population (European Commission, 2007).

Ethernet network interface card (NIC) or modem with a speed of at least 28,800 bps A CD-ROM drive For system requirements for AutoCAD 2006 and AutoCAD LT 2006, see Appendix A.

I am going to throw you a curve ball here. Many of you will want to produce a show in more than one audio format. As I said earlier, I create audio files in MP3, WMA, and OGG format. Luckily, Brandon Fuller, when he created this plug-in, thought about that too and designed a way in which we could designate a specific extension to be included in the RSS 2.0 output file. For a podcatcher to work properly, you should never have more than one enclosure element in the RSS 2.0 file per weblog post. Listing 11-3 shows an actual RSS 2.0 file with an enclosure element. I will break this down a little further so you understand.

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An oligonucleotide can be designed and synthesized to hybridize speci cally to a particular piece of DNA or RNA (e.g. part of a gene, a ribosome or a virus) according to the rules of Watson Crick base pairing. When coupled with the use of in situ polymerase chain reaction (which allows ampli cation of a target sequence), this technique gives a sensitive and speci c means of locating DNA or RNA in cells. If pyrimidine nucleotides labelled with uorescein are incorporated into the oligonucleotide, double-stranded hybrids with the target sequence will be visible. Non- uorescent oligonucleotide labels such as digoxigenin and biotin can be used in the same way but these must be detected with the aid of uorescently-labelled antibodies as described above. This technique has been used in a wide range of studies in biochemistry including elucidation of chromatin structure, the detection of viruses and in positional cloning (i.e. the location of defective genes in chromosomes by phenotype mapping without prior knowledge of the functional basis of disease). In situ hybridization can also be used in combination with immunostaining to demonstrate, for example, colocalization of mRNA and translated protein or to identify the location of viruses in viral infections.

changed by rotating one of the prisms about its optical axis by an amount f=2. The interferometer proposed by Armitage and Lohmann is illustrated in Figure 5.30. The wavefront under test is collimated by the lens L1 or a pinhole is used if L1 is the lens to be tested. The pair of lenses L2 and L3 forms an image of the entrance pupil at L1 on the observing screen. To obtain good coherence, the state of polarization of both beams must be identical, but this is not so when the rotating roof prism is rotated at an angle f/2, because the roof prisms modify the state of polarization according to their positions. To solve this problem, a polarization coupling is used as follows. Two polarizers are included, one before and one after the interferometer, both at 0 . Also, two quarter-wave plates are placed between the main interferometer body and the

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